The Mouse Pro-ANP (Pro-Atrial Natriuretic Peptide) ELISA Kit is specially designed for the accurate quantification of pro-atrial natriuretic peptide levels in mouse serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, guaranteeing dependable and consistent results, making it perfect for a variety of research purposes.Pro-atrial natriuretic peptide is a key hormone involved in regulating blood pressure and fluid balance in the body.
It is particularly important in cardiovascular health and can serve as a vital biomarker for studying cardiovascular diseases, heart failure, and other related conditions. This ELISA kit provides a valuable tool for researchers looking to explore the role of pro-ANP in various biological processes and potentially develop new treatment strategies.
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse Pro-ANP. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse Pro-ANP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse Pro-ANP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse Pro-ANP. You can calculate the concentration of Mouse Pro-ANP in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
84-97
94-110
93-109
Average (%)
90
100
100
1:4
Range (%)
92-104
84-94
87-99
Average (%)
97
89
94
1:8
Range (%)
93-104
85-99
83-98
Average (%)
99
90
89
1:16
Range (%)
89-104
83-95
85-96
Average (%)
95
90
91
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-104
98
EDTA plasma (n=5)
91-105
96
Cell culture media (n=5)
84-96
91
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
47.59
133.22
411.8
43.89
130.39
408.37
Standard deviation
2.77
5.73
16.68
2.5
5.7
15.52
C V (%)
5.82
4.3
4.05
5.7
4.37
3.8
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse Pro-ANP concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse Pro-ANP in samples. No significant cross-reactivity or interference between Mouse Pro-ANP and analogues was observed.
