SynonymsPRO-C,VKDPC,PC,Vitamin K Dependent Protein C,Inactivator Of Coagulation Factors Va And VIIIa,Autoprothrombin IIA,Blood coagulation factor XIV,Activation peptide
ReactivityMouse
Sample TypePlasma
SKU:
MODL01091
UPC:
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P33587
Sensitivity:
14.1pg/mL
Range:
31.25-2000pg/mL
ELISA Type:
Sandwich
Synonyms:
PRO-C,VKDPC,PC,Vitamin K Dependent Protein C,Inactivator Of Coagulation Factors Va And VIIIa,Autoprothrombin IIA,Blood coagulation factor XIV,Activation peptide
The Mouse Protein C (PROC) ELISA Kit is specifically designed for the precise quantification of protein C levels in mouse serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and accuracy, allowing for consistent and dependable results, which makes it perfect for various research purposes.Protein C is a vital component of the blood coagulation system, playing a crucial role in regulating blood clotting. Dysregulation of protein C has been linked to various coagulation disorders and thrombotic events, underscoring its importance as a biomarker in studying these conditions and developing targeted therapies.
Overall, the Mouse Protein C (PROC) ELISA Kit provides researchers with a powerful tool to investigate the role of protein C in health and disease in mouse models, advancing our understanding of coagulation disorders and potential therapeutic interventions.
Product Name:
Mouse Protein C (PROC) ELISA Kit
Product Code:
MODL01091
Size:
96 Assays
Target:
PROC
Synonyms:
PRO-C, VKDPC, PC, Vitamin K Dependent Protein C, Inactivator Of Coagulation Factors Va And VIIIa, Autoprothrombin IIA, Blood coagulation factor XIV, Activation peptide
Detection Method:
Sandwich
Range:
31.2-2,000pg/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
