Mouse Protein C Receptor, Endothelial (PROCR) ELISA Kit
The Mouse PROCR (Protein C Receptor, Endothelial) ELISA Kit is designed for the accurate detection of PROCR levels in mouse serum, plasma, and cell culture supernatants. This kit features high sensitivity and specificity, ensuring reliable and reproducible results, making it ideal for a wide range of research applications.PROCR is a key protein involved in maintaining vascular integrity and regulating blood coagulation. It plays a critical role in processes such as endothelial cell function, inflammation, and angiogenesis, making it an important target for studying vascular disorders, thrombosis, and other related conditions.
With this ELISA kit, researchers can quantitatively assess PROCR levels in mouse samples, offering valuable insights into the molecular mechanisms underlying vascular biology and potentially identifying new therapeutic targets for various cardiovascular and inflammatory diseases.
Product Name:
Mouse Protein C Receptor, Endothelial (PROCR) ELISA Kit
Product Code:
MODL01092
Size:
96 Assays
Target:
PROCR
Synonyms:
CD201, CCCA, CCD41, BA4 2O4.2, EPCR, Endothelial Protein C Receptor, Cell Cycle, Centrosome-Associated Protein, Activated Protein C Receptor
Detection Method:
Sandwich
Range:
78.1-5,000pg/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
