Mouse Protein Kinase, DNA Activated, Catalytic Polypeptide (PRKDC) ELISA Kit
The Mouse PRKDC (Protein Kinase, DNA-Activated, Catalytic Polypeptide) ELISA Kit is specifically designed for the quantitative detection of PRKDC levels in mouse samples including serum, plasma, and tissue homogenates. This kit offers exceptional sensitivity and specificity, ensuring reliable and consistent results for a variety of research applications.PRKDC is a key protein kinase responsible for DNA repair and maintenance of genomic stability. Dysregulation of PRKDC has been linked to various diseases including cancer, immunodeficiency, and premature aging.
Therefore, accurate measurement of PRKDC levels is crucial for understanding its role in disease progression and developing targeted therapies.The Mouse PRKDC ELISA Kit provides a valuable tool for researchers studying DNA damage response, genome stability, and related pathways in mouse models. With its high performance and easy-to-use format, this kit is a valuable asset for investigating the functional significance of PRKDC in physiological and pathological conditions.
Product Name:
Mouse Protein Kinase, DNA Activated, Catalytic Polypeptide (PRKDC) ELISA Kit
Product Code:
MODL01087
Size:
96 Assays
Target:
PRKDC
Synonyms:
HYRC, HYRC1, DNPK1, p350, DNAPK, XRCC7, DNA-PKcs
Detection Method:
Sandwich
Range:
0.312-20ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
