The Mouse PYY (Peptide YY) ELISA Kit is a highly sensitive and specific assay designed for the accurate measurement of PYY levels in mouse serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it ideal for various research applications.Peptide YY is a peptide hormone that plays a crucial role in regulating appetite and energy balance. It is also involved in gut motility and absorption of nutrients. Dysregulation of PYY levels has been linked to conditions such as obesity, diabetes, and gastrointestinal disorders, making it an important biomarker for studying these conditions and developing potential therapeutic interventions.
The Mouse PYY ELISA Kit offers researchers a valuable tool for studying the role of PYY in various physiological and pathological processes, ultimately advancing our understanding of metabolic and gastrointestinal health.
Product Name:
Mouse PYY (Peptide YY) ELISA Kit
SKU:
MOES01678
Target:
Mouse PYY (Peptide YY)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse PYY. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse PYY and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse PYY, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse PYY. You can calculate the concentration of Mouse PYY in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
96-110
90-107
93-108
Average (%)
104
97
99
1:4
Range (%)
87-99
84-99
82-93
Average (%)
93
91
88
1:8
Range (%)
88-103
81-91
89-102
Average (%)
94
86
94
1:16
Range (%)
87-97
80-92
85-98
Average (%)
92
87
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-103
95
EDTA plasma (n=5)
90-103
96
Cell culture media (n=5)
87-103
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
45.71
159.95
339.32
41.31
153.09
353.76
Standard deviation
2.3
9.12
11.81
2.17
7.15
19.35
C V (%)
5.03
5.7
3.48
5.25
4.67
5.47
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse PYY concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse PYY in samples. No significant cross-reactivity or interference between Mouse PYY and analogues was observed.
