The Mouse R-spondin 1 (RSPO1) ELISA Kit is a valuable tool for the quantitative measurement of RSPO1 levels in mouse serum, plasma, and tissue homogenates. With its high sensitivity and specificity, this kit provides accurate and reliable results for various research applications.RSPO1 is a critical protein known for its role in regulating Wnt signaling pathways, which are involved in embryonic development, tissue regeneration, and stem cell proliferation. Dysregulation of RSPO1 has been linked to various diseases, including cancer and gastrointestinal disorders, making it a key target for therapeutic interventions and biomarker studies.
Whether investigating the mechanisms of RSPO1 in normal physiological processes or exploring its potential as a therapeutic target, the Mouse RSPO1 ELISA Kit offers precise and efficient detection of RSPO1 levels in biological samples. Trust this kit to advance your research and uncover new insights into the complex functions of RSPO1 in health and disease.
Product Name:
Mouse R-Spondin 1 (RSPO1) ELISA Kit
Product Code:
MODL01136
Size:
96 Assays
Target:
RSPO1
Synonyms:
RSPONDIN, Roof plate-specific spondin-1
Detection Method:
Sandwich
Range:
15.6-1,000pg/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
