Mouse RANk (Receptor Activator of Nuclear Factor Kappa B) CLIA Kit
The Mouse Rank Receptor Activator of Nuclear Factor Kappa B CLIA Kit is a highly sensitive and specific assay designed for the precise measurement of Rank Receptor Activator of Nuclear Factor Kappa B levels in mouse serum, plasma, and tissue homogenates. This kit offers reliable and reproducible results, making it an excellent choice for a variety of research applications.The Rank Receptor Activator of Nuclear Factor Kappa B is a key molecule involved in the regulation of bone metabolism, immune response, and inflammation.
Dysregulation of this pathway has been linked to various diseases, including osteoporosis, arthritis, and cancer. This kit provides a valuable tool for studying the role of Rank Receptor Activator of Nuclear Factor Kappa B in these conditions and exploring potential therapeutic interventions.
Product Name:
Mouse RANk (Receptor Activator of Nuclear Factor Kappa B) CLIA Kit
SKU:
MOES00353
Target:
Mouse RANk (Receptor Activator of Nuclear Factor Kappa B)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse RANκ. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse RANκ and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse RANκ, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse RANκ. You can calculate the concentration of Mouse RANκ in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
101-116
102-118
93-107
Average (%)
108
109
98
1:4
Range (%)
100-114
87-98
98-111
Average (%)
106
92
105
1:8
Range (%)
93-110
104-118
90-104
Average (%)
100
110
98
1:16
Range (%)
91-102
95-112
98-117
Average (%)
96
103
106
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
99-114
106
EDTA plasma (n=5)
85-96
90
Cell culture media (n=5)
94-107
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
53.73
137.63
468.82
54.92
145.53
496.13
Standard deviation
4.88
15.21
28.83
5.87
13.55
44.4
C V (%)
9.08
11.05
6.15
10.69
9.31
8.95
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse RANκ concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse RANκ in samples. No significant cross-reactivity or interference between Mouse RANκ and analogues was observed.
