Mouse Receptor Activator Of Nuclear Factor Kappa B Ligand (RANkL) ELISA Kit
The Mouse Receptor Activator of Nuclear Factor Kappa B Ligand (RANKL) ELISA Kit is a powerful tool for researchers looking to accurately detect RANKL levels in mouse serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit delivers reliable and reproducible results, making it suitable for a wide range of research applications.RANKL is a key cytokine involved in bone metabolism, regulating osteoclast differentiation and function.
Dysregulation of RANKL has been linked to various bone disorders, such as osteoporosis and rheumatoid arthritis, making it a critical biomarker for studying bone health and developing potential treatments.By using the Mouse RANKL ELISA Kit, researchers can gain valuable insights into the role of RANKL in bone physiology and pathology, paving the way for advancements in bone-related research and therapeutic strategies.
Product Name:
Mouse Receptor Activator Of Nuclear Factor Kappa B Ligand (RANkL) ELISA Kit
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
87-100
93
EDTA plasma (n=5)
89-99
94
Heparin plasma (n=5)
90-98
94
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
