Mouse RANkL (Receptor Activator of Nuclear Factor Kappa B Ligand) CLIA Kit
The Mouse RANKL (Receptor Activator of Nuclear Factor Kappa-B Ligand) CLIA Kit is a specialized assay kit designed for the accurate detection of RANKL levels in mouse serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reliable results for research applications.RANKL is a key protein involved in the regulation of bone metabolism and immune response, playing a crucial role in conditions such as osteoporosis, arthritis, and inflammatory diseases.
The Mouse RANKL CLIA Kit provides researchers with a valuable tool for studying these conditions and exploring potential therapeutic interventions.With its user-friendly format and robust performance, the Mouse RANKL CLIA Kit is an essential resource for investigating the role of RANKL in mouse models and advancing our understanding of bone biology and immune system function.
Product Name:
Mouse RANkL (Receptor Activator of Nuclear Factor Kappa B Ligand) CLIA Kit
SKU:
MOES00346
Target:
Mouse RANkL (Receptor Activator of Nuclear Factor Kappa B Ligand)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse RANκL. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse RANκL and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse RANκL, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse RANκL. You can calculate the concentration of Mouse RANκL in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
84-96
101-117
93-108
Average (%)
90
109
99
1:4
Range (%)
87-100
89-104
86-99
Average (%)
93
96
93
1:8
Range (%)
90-103
90-105
100-113
Average (%)
97
96
106
1:16
Range (%)
96-109
85-98
94-111
Average (%)
103
90
102
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
95-107
100
EDTA plasma (n=5)
88-101
93
Cell culture media (n=5)
100-115
108
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
24.81
66.42
172.04
23.64
69.61
174.97
Standard deviation
2.06
7.72
19.03
2.48
6.2
13.32
C V (%)
8.3
11.62
11.06
10.49
8.91
7.61
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse RANκL concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse RANκL in samples. No significant cross-reactivity or interference between Mouse RANκL and analogues was observed.
