Mouse Receptor I For The Fc Region Of Immunoglobulin G (FcgRI) ELISA Kit
The Mouse Receptor I for the Fc Region of Immunoglobulin G (FcγRI) ELISA Kit is a cutting-edge tool for the accurate measurement of FcγRI levels in mouse samples, including serum, plasma, and cell culture supernatants. With its exceptional sensitivity and specificity, this kit delivers dependable and consistent results, making it an indispensable resource for a wide range of research investigations.FcγRI is a pivotal receptor involved in the immune response, mediating the recognition and activation of immune cells in response to immunoglobulin G (IgG) antibodies. Its dysregulation has been linked to various autoimmune diseases, inflammatory conditions, and infectious diseases, highlighting its significance as a key target for therapeutic interventions.
This ELISA kit provides researchers with a comprehensive tool for studying the role of FcγRI in immune function and disease pathogenesis, paving the way for the development of novel therapeutic strategies and diagnostic approaches. Trust in the Mouse Receptor I for Fc Region of Immunoglobulin G (FcγRI) ELISA Kit for accurate and precise measurements in your research endeavors.
Product Name:
Mouse Receptor I For The Fc Region Of Immunoglobulin G (FcgRI) ELISA Kit
Product Code:
MODL00465
Size:
96 Assays
Target:
FcgRI
Synonyms:
CD64, FCGR1, FCGR1A, FCGR1B, FCGR1C, FcgRIA, CD64A, FcgRIB, CD64B, FcgRIC, CD64C, High affinity immunoglobulin gamma Fc receptor I
Detection Method:
Sandwich
Range:
0.625-40ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
