Mouse RUNX2 (Runt Related Transcription Factor 2) CLIA Kit
The Mouse Runx2 (Runt-Related Transcription Factor 2) CLIA Kit is specifically designed for the measurement of Runx2 levels in mouse serum, plasma, and tissue lysates. This kit offers exceptional sensitivity and specificity, ensuring accurate and dependable results for various research applications.Runx2 is a key transcription factor essential for osteoblast differentiation and bone formation. It plays a critical role in skeletal development, fracture healing, and bone remodeling processes. Dysregulation of Runx2 has been linked to skeletal disorders such as osteoporosis and skeletal deformities, making it a valuable target for studying bone metabolism and therapeutic interventions.
With its reliable performance and ease of use, the Mouse Runx2 CLIA Kit is an invaluable tool for researchers studying bone biology, skeletal development, and related disorders in mouse models. Get precise and quantitative measurements of Runx2 levels with this advanced kit, enabling insightful discoveries and advancements in the field of bone research.
Product Name:
Mouse RUNX2 (Runt Related Transcription Factor 2) CLIA Kit
SKU:
MOES00519
Target:
Mouse RUNX2 (Runt Related Transcription Factor 2)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse RUNX2. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse RUNX2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse RUNX2, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse RUNX2. You can calculate the concentration of Mouse RUNX2 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
101-118
101-118
94-107
Average (%)
109
110
100
1:4
Range (%)
88-101
98-116
85-95
Average (%)
93
106
90
1:8
Range (%)
88-102
91-103
90-101
Average (%)
95
97
95
1:16
Range (%)
94-105
90-105
100-113
Average (%)
100
97
106
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
95-109
100
EDTA plasma (n=5)
84-96
91
Cell culture media (n=5)
96-106
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
189.38
366.44
1539.99
182.15
336.88
1412.93
Standard deviation
17.14
40.16
158.93
18.98
28.5
104.56
C V (%)
9.05
10.96
10.32
10.42
8.46
7.4
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse RUNX2 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse RUNX2 in samples. No significant cross-reactivity or interference between Mouse RUNX2 and analogues was observed.
