Mouse S100 Calcium Binding Protein A11 (S100A11) ELISA Kit
The Mouse S100 Calcium Binding Protein A11 (S100A11) ELISA Kit is specifically designed for the accurate quantification of S100A11 levels in mouse serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, guaranteeing precise and consistent results, making it an ideal tool for various research applications.S100A11 is a pivotal protein that binds calcium and is involved in various cellular processes, including cell growth, differentiation, and inflammation.
It plays a vital role in conditions such as cancer, inflammation, and dermatological disorders, making it a valuable biomarker for studying these conditions and developing potential therapies.Overall, the Mouse S100 Calcium Binding Protein A11 (S100A11) ELISA Kit provides researchers with a reliable and efficient method for measuring S100A11 levels in mouse biological samples, advancing our understanding of the protein's function and its implications in disease pathology.
Product Name:
Mouse S100 Calcium Binding Protein A11 (S100A11) ELISA Kit
Product Code:
MODL01142
Size:
96 Assays
Target:
S100A11
Synonyms:
S100-A11, S100C, MLN70, Protein S100-C, Calgizzarin, Metastatic lymph node gene 70 protein, Protein S100-A11, N-terminally processed
Detection Method:
Sandwich
Range:
62.5-4,000pg/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-100
91
EDTA plasma (n=5)
90-98
94
Heparin plasma (n=5)
89-102
95
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
