Mouse S100A10 (S100 Calcium-binding Protein A10) CLIA Kit
The Mouse S100A10 (S100 Calcium Binding Protein A10) CLIA Kit is a reliable assay designed for the precise measurement of S100A10 levels in mouse samples such as serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, guaranteeing accurate and consistent results for various research applications.S100A10 is a key calcium-binding protein that plays a crucial role in various cellular processes, including cell proliferation, apoptosis, and intracellular signaling.
Its dysregulation has been associated with several diseases, including cancer, inflammatory disorders, and neurodegenerative conditions, making it a valuable biomarker for studying these pathological conditions and developing potential therapeutic interventions. With its easy-to-use format and robust performance, the Mouse S100A10 CLIA Kit is a valuable tool for researchers studying the role of S100A10 in health and disease.
Product Name:
Mouse S100A10 (S100 Calcium-binding Protein A10) CLIA Kit
SKU:
MOES00610
Target:
Mouse S100A10 (S100 Calcium-binding Protein A10)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse S100A10. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse S100A10 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse S100A10, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse S100A10. You can calculate the concentration of Mouse S100A10 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
99-113
92-107
89-102
Average (%)
106
99
95
1:4
Range (%)
100-116
87-98
96-109
Average (%)
108
92
103
1:8
Range (%)
101-117
91-104
96-110
Average (%)
108
98
102
1:16
Range (%)
92-105
86-100
90-102
Average (%)
98
91
97
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-107
99
EDTA plasma (n=5)
90-102
97
Cell culture media (n=5)
94-111
102
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
190.63
422.91
1639.01
176.35
395.6
1698.69
Standard deviation
23.18
45.97
170.62
17.48
36.2
190.42
C V (%)
12.16
10.87
10.41
9.91
9.15
11.21
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse S100A10 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse S100A10 in samples. No significant cross-reactivity or interference between Mouse S100A10 and analogues was observed.
