Mouse S100A6 (S100 Calcium Binding Protein A6) CLIA Kit
The Mouse S100A6 (S100 Calcium Binding Protein A6) CLIA Kit is specifically designed for the accurate measurement of S100A6 levels in mouse serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit provides reliable and reproducible results, making it perfect for a variety of research applications.S100A6 is a calcium-binding protein that plays a crucial role in regulating cell growth, differentiation, and migration. It is involved in various cellular processes and has been implicated in conditions such as cancer and inflammatory diseases.
By accurately measuring S100A6 levels, researchers can gain valuable insights into the mechanisms underlying these diseases and potentially identify new therapeutic targets.Overall, the Mouse S100A6 CLIA Kit provides researchers with a valuable tool for studying the role of S100A6 in health and disease, offering a reliable and efficient method for quantifying S100A6 levels in mouse samples.
Product Name:
Mouse S100A6 (S100 Calcium Binding Protein A6) CLIA Kit
SKU:
MOES00523
Target:
Mouse S100A6 (S100 Calcium Binding Protein A6)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse S100A6. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse S100A6 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse S100A6, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse S100A6. You can calculate the concentration of Mouse S100A6 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
102-117
92-106
99-113
Average (%)
108
99
107
1:4
Range (%)
94-110
95-107
99-111
Average (%)
101
101
106
1:8
Range (%)
88-99
86-103
101-115
Average (%)
94
94
108
1:16
Range (%)
85-98
89-102
85-100
Average (%)
90
95
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
97-112
103
EDTA plasma (n=5)
94-112
102
Cell culture media (n=5)
88-98
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
96.07
315.86
833.11
90.68
335.18
863.95
Standard deviation
8.7
25.27
78.98
9.6
30.94
81.99
C V (%)
9.06
8.0
9.48
10.59
9.23
9.49
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse S100A6 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse S100A6 in samples. No significant cross-reactivity or interference between Mouse S100A6 and analogues was observed.
