The Mouse SCFR (Stem Cell Factor Receptor) CLIA Kit is specifically designed for the quantitative detection of SCFR levels in mouse serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit provides accurate and reliable results for a variety of research applications.SCFR, also known as c-Kit, is a receptor tyrosine kinase that plays a critical role in various cellular functions, including cell survival, proliferation, and differentiation.
Dysregulation of SCFR has been associated with a range of diseases, such as cancer, hematopoietic disorders, and inflammatory conditions, making it a valuable biomarker for studying these pathologies and potential therapeutic interventions.Overall, the Mouse SCFR CLIA Kit offers a comprehensive and effective solution for researchers seeking to investigate SCFR levels in mouse samples and explore its implications in various physiological and pathological processes.
Product Name:
Mouse SCFR (Stem Cell Factor Receptor) CLIA Kit
SKU:
MOES00538
Target:
Mouse SCFR (Stem Cell Factor Receptor)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse SCFR. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse SCFR and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse SCFR, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse SCFR. You can calculate the concentration of Mouse SCFR in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
90-103
94-109
93-106
Average (%)
96
101
99
1:4
Range (%)
100-114
93-105
93-107
Average (%)
105
99
99
1:8
Range (%)
90-102
93-107
90-101
Average (%)
96
101
95
1:16
Range (%)
88-102
85-98
91-105
Average (%)
95
92
97
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
99-114
105
EDTA plasma (n=5)
88-100
94
Cell culture media (n=5)
91-104
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
108.95
177.38
937.78
105.39
169.56
949.5
Standard deviation
13.66
16.05
60.49
8.86
18.97
85.93
C V (%)
12.54
9.05
6.45
8.41
11.19
9.05
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse SCFR concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse SCFR in samples. No significant cross-reactivity or interference between Mouse SCFR and analogues was observed.
