The Mouse Stem Cell Factor Receptor (SCFR) ELISA Kit from AssayGenie is a cutting-edge tool designed for the precise detection of SCFR levels in mouse serum, plasma, and cell culture supernatants. With its superior sensitivity and specificity, this kit delivers accurate and consistent results, making it ideal for a broad range of research applications.SCFR, also known as c-Kit or CD117, is a key receptor involved in the regulation of stem cell proliferation and differentiation. Its dysregulation has been linked to various diseases, making it a crucial biomarker for investigating conditions such as cancer, hematopoiesis, and developmental disorders.
The Mouse SCFR ELISA Kit offers researchers a reliable and efficient way to study SCFR expression levels and explore its role in health and disease.Key Features:- Detection of SCFR levels in mouse samples with high sensitivity and specificity- Suitable for serum, plasma, and cell culture supernatants- Reliable and reproducible results for consistent data analysis- Ideal for research on stem cell biology, cancer, and other related fieldsChoose the Mouse SCFR ELISA Kit from AssayGenie to unlock new insights into stem cell biology and disease mechanisms. Order now and elevate your research to the next level.
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
