The Mouse SDC1 (Syndecan-1) ELISA Kit is specifically designed for the accurate detection of syndecan-1 levels in mouse serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring reliable and reproducible results for a variety of research applications.Syndecan-1 is a key cell surface proteoglycan involved in cell adhesion and signaling, playing a critical role in cell proliferation, migration, and differentiation.
Dysregulation of syndecan-1 has been implicated in various diseases, including cancer, inflammation, and fibrosis, making it a valuable biomarker for studying these conditions and developing targeted therapies.Overall, the Mouse SDC1 (Syndecan-1) ELISA Kit provides researchers with a powerful tool for investigating the role of syndecan-1 in mouse models and advancing our understanding of its biological functions and potential therapeutic implications.
Product Name:
Mouse SDC1 (Syndecan 1) ELISA Kit
SKU:
MOES01738
Target:
Mouse SDC1 (Syndecan 1)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse SDC1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse SDC1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse SDC1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse SDC1. You can calculate the concentration of Mouse SDC1 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
85-97
91-104
87-99
Average (%)
92
97
94
1:4
Range (%)
94-106
87-101
87-98
Average (%)
99
93
92
1:8
Range (%)
95-109
80-92
87-100
Average (%)
100
86
94
1:16
Range (%)
92-104
81-94
85-98
Average (%)
98
87
90
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-100
94
EDTA plasma (n=5)
92-106
100
Cell culture media (n=5)
90-106
97
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.46
1.0
4.46
0.48
1.1
4.68
Standard deviation
0.03
0.04
0.2
0.03
0.06
0.16
C V (%)
6.52
4.0
4.48
6.25
5.45
3.42
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse SDC1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse SDC1 in samples. No significant cross-reactivity or interference between Mouse SDC1 and analogues was observed.
