The Mouse Secretagogin (SCGN) ELISA Kit is a valuable tool for researchers looking to accurately measure secretagogin levels in mouse serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, providing dependable and consistent results for a variety of research studies.Secretagogin is a key protein involved in regulating hormone secretion and calcium signaling in the body. It plays a crucial role in various physiological processes, including metabolism, neuronal function, and stress response.
Understanding secretagogin levels can provide valuable insights into conditions such as diabetes, neurodegenerative diseases, and metabolic disorders, making it a valuable biomarker for disease research and therapeutic development.Overall, the Mouse Secretagogin (SCGN) ELISA Kit offers a reliable and efficient way to study secretagogin levels in mouse samples, making it an essential tool for researchers in the field of molecular biology and biomedical research.
Product Name:
Mouse Secretagogin (SCGN) ELISA Kit
Product Code:
MODL01158
Size:
96 Assays
Target:
SCGN
Synonyms:
SECRET, SEGN, CALBL, Calbindin-Like, Secretagogin,EF-Hand Calcium Binding Protein
Detection Method:
Sandwich
Range:
78.1-5,000pg/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
