The Mouse Secretogranin II (SCG2) ELISA Kit is a reliable and sensitive assay designed for the precise measurement of secretogranin II levels in mouse serum, plasma, and cell culture supernatants. This highly specific kit ensures accurate and reproducible results, making it an ideal tool for various research applications.Secretogranin II is a key protein involved in regulated exocytosis and neuroendocrine secretion, playing a crucial role in neurotransmitter release and hormone secretion. Its dysregulation has been linked to various diseases, including diabetes, neurodegenerative disorders, and cancer, making it a valuable biomarker for understanding these conditions and developing potential treatments.
With its high sensitivity and specificity, the Mouse Secretogranin II (SCG2) ELISA Kit is a valuable resource for researchers studying neuroendocrine function, exocytosis, and related diseases in mouse models. Get reliable and accurate results with this advanced kit from Assay Genie.
Product Name:
Mouse Secretogranin II (SCG2) ELISA Kit
Product Code:
MODL01157
Size:
96 Assays
Target:
SCG2
Synonyms:
SN, SgII, CHGC, Chromogranin C, Secretoneurin
Detection Method:
Sandwich
Range:
0.312-20ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
