The Mouse SELE (E-selectin) ELISA Kit is a specialized assay crafted for the quantitative detection of E-selectin in various mouse biological samples. E-selectin, a cell adhesion molecule, plays a crucial role in mediating leukocyte-endothelial interactions, which are essential for the immune response and the inflammatory process. This ELISA kit boasts exceptional sensitivity and specificity, ensuring accurate and reproducible results in research studies aiming to understand the role of E-selectin in inflammatory pathways, vascular biology, and immune-related processes.
Manufactured under stringent quality control standards, the kit delivers robust performance and ease of use, making it a reliable choice for investigators exploring the molecular mechanisms of inflammation and immune cell recruitment.
Product Name:
Mouse SELE (E-selectin) ELISA Kit
SKU:
AEES00222
Target:
Mouse SELE (E-selectin)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse SELE. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse SELE and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse SELE, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse SELE. You can calculate the concentration of Mouse SELE in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
101-113
95-109
101-109
Average (%)
105
103
104
1:4
Range (%)
96-105
92-101
86-96
Average (%)
100
95
92
1:8
Range (%)
88-99
102-108
92-99
Average (%)
96
105
95
1:16
Range (%)
89-98
87-99
94-105
Average (%)
95
92
98
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
84-96
92
EDTA plasma (n=5)
96-107
101
Cell culture media (n=5)
85-99
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.9
2.28
9.27
0.92
2.13
8.61
Standard deviation
0.05
0.11
0.41
0.05
0.1
0.35
C V (%)
5.56
4.82
4.42
5.43
4.69
4.07
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse SELE concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse SELE in samples. No significant cross-reactivity or interference between Mouse SELE and analogues was observed.
