The Mouse SESN1 (Sestrin-1) CLIA Kit is a highly sensitive and specific assay designed for the quantitative detection of SESN1 levels in mouse serum, plasma, and cell culture supernatants. This kit provides accurate and reproducible results, making it an excellent tool for researchers studying the role of Sestrin-1 in various biological processes.Sestrin-1 is a key regulator of cellular metabolism and stress response, playing a crucial role in pathways related to aging, obesity, and muscle regeneration.
By measuring SESN1 levels, scientists can gain valuable insights into the mechanisms underlying these processes and identify potential therapeutic targets.Overall, the Mouse SESN1 CLIA Kit offers a reliable and efficient solution for investigating the functions of Sestrin-1 in a wide range of experimental settings, making it a valuable addition to any research laboratory.
Product Name:
Mouse SESN1 (Sestrin 1) CLIA Kit
SKU:
MOES00528
Target:
Mouse SESN1 (Sestrin 1)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse SESN1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse SESN1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse SESN1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse SESN1. You can calculate the concentration of Mouse SESN1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
95-108
89-102
91-105
Average (%)
102
96
99
1:4
Range (%)
85-97
101-119
88-104
Average (%)
91
108
95
1:8
Range (%)
99-111
103-118
94-108
Average (%)
104
110
101
1:16
Range (%)
92-109
96-109
92-107
Average (%)
99
102
98
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
99-115
106
EDTA plasma (n=5)
95-112
103
Cell culture media (n=5)
93-104
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
95.0
203.25
724.75
99.74
212.86
782.89
Standard deviation
9.16
19.59
60.95
8.33
22.24
75.39
C V (%)
9.64
9.64
8.41
8.35
10.45
9.63
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse SESN1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse SESN1 in samples. No significant cross-reactivity or interference between Mouse SESN1 and analogues was observed.
