The Mouse SESN3 (Sestrin-3) CLIA Kit is a highly sensitive and specific assay designed for the detection of SESN3 levels in mouse serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it ideal for various research applications.SESN3, also known as Sestrin-3, is a critical protein involved in cellular stress response and regulating metabolism. It plays a crucial role in various physiological processes and has been implicated in conditions such as cancer, diabetes, and aging.
The Mouse SESN3 CLIA Kit is essential for studying the role of SESN3 in these conditions and developing potential therapeutic interventions.Overall, the Mouse SESN3 CLIA Kit offers researchers a valuable tool for accurately measuring SESN3 levels in mouse samples, allowing for a better understanding of its biological functions and potential implications in disease pathogenesis.
Product Name:
Mouse SESN3 (Sestrin 3) CLIA Kit
SKU:
MOES00530
Target:
Mouse SESN3 (Sestrin 3)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse SESN3. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse SESN3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse SESN3, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse SESN3. You can calculate the concentration of Mouse SESN3 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
88-104
102-120
90-102
Average (%)
96
109
97
1:4
Range (%)
98-115
102-114
85-97
Average (%)
106
108
90
1:8
Range (%)
85-98
98-112
88-101
Average (%)
92
104
93
1:16
Range (%)
93-107
100-114
84-96
Average (%)
100
105
90
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
97-115
105
EDTA plasma (n=5)
96-112
104
Cell culture media (n=5)
101-114
107
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
109.56
223.18
965.77
101.19
206.55
1048.56
Standard deviation
11.66
22.14
94.74
9.31
15.1
82.1
C V (%)
10.64
9.92
9.81
9.2
7.31
7.83
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse SESN3 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse SESN3 in samples. No significant cross-reactivity or interference between Mouse SESN3 and analogues was observed.
