The Mouse SHBG (Sex Hormone Binding Globulin) CLIA Kit is specifically designed for the accurate quantification of SHBG levels in mouse serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity, precision, and specificity, ensuring reliable and reproducible results for a variety of research applications.SHBG is a critical protein that binds and regulates the bioavailability of sex hormones such as testosterone and estrogen. Dysregulation of SHBG levels has been linked to various reproductive, metabolic, and endocrine disorders, making it a valuable biomarker for studying these conditions and developing potential therapeutic interventions.
With the Mouse SHBG CLIA Kit, researchers can efficiently measure SHBG levels in mouse samples, providing valuable insights into hormonal homeostasis and potential disease mechanisms. Its user-friendly format, high performance, and broad sample compatibility make it an indispensable tool for investigating the role of SHBG in health and disease.
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse SHBG. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse SHBG and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse SHBG, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse SHBG. You can calculate the concentration of Mouse SHBG in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
99-114
98-114
104-119
Average (%)
107
105
110
1:4
Range (%)
87-100
101-116
93-108
Average (%)
93
107
100
1:8
Range (%)
100-117
93-107
100-118
Average (%)
107
98
108
1:16
Range (%)
87-101
93-106
88-101
Average (%)
93
100
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
102-117
108
EDTA plasma (n=5)
85-98
92
Cell culture media (n=5)
85-97
91
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
43.93
86.48
335.94
44.06
89.13
351.05
Standard deviation
4.36
10.23
36.32
5.53
6.91
22.15
C V (%)
9.92
11.83
10.81
12.55
7.75
6.31
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse SHBG concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse SHBG in samples. No significant cross-reactivity or interference between Mouse SHBG and analogues was observed.
