Mouse SOCS3 (Suppressors Of Cytokine Signaling 3) CLIA Kit
The Mouse SOCS3 (Suppressors of Cytokine Signaling 3) CLIA Kit is specifically designed for the quantitative measurement of SOCS3 levels in mouse serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring accurate and reliable results for various research applications.SOCS3 is an important protein that regulates the signaling pathways involved in immune response and inflammation. It plays a key role in modulating cytokine signaling and controlling immune cell activation.
Dysregulation of SOCS3 has been implicated in various diseases, including cancer, inflammatory disorders, and autoimmune diseases, making it a valuable biomarker for understanding these conditions and developing potential therapeutic strategies.Overall, the Mouse SOCS3 CLIA Kit provides researchers with a powerful tool for studying the role of SOCS3 in immune regulation and disease pathology, offering insights into potential treatment targets and biomarkers for diagnostic purposes.
Product Name:
Mouse SOCS3 (Suppressors Of Cytokine Signaling 3) CLIA Kit
SKU:
MOES00541
Target:
Mouse SOCS3 (Suppressors Of Cytokine Signaling 3)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse SOCS3. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse SOCS3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse SOCS3, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse SOCS3. You can calculate the concentration of Mouse SOCS3 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
95-110
100-114
87-99
Average (%)
103
108
93
1:4
Range (%)
93-107
92-104
96-112
Average (%)
100
99
102
1:8
Range (%)
96-110
96-110
92-105
Average (%)
101
103
100
1:16
Range (%)
93-109
96-111
102-117
Average (%)
100
101
108
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-106
99
EDTA plasma (n=5)
98-114
104
Cell culture media (n=5)
97-112
102
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
98.36
249.1
946.3
89.1
238.88
886.88
Standard deviation
9.6
24.61
73.62
7.29
26.56
76.54
C V (%)
9.76
9.88
7.78
8.18
11.12
8.63
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse SOCS3 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse SOCS3 in samples. No significant cross-reactivity or interference between Mouse SOCS3 and analogues was observed.
