Mouse Suppressors Of Cytokine Signaling 1 (SOCS1) ELISA Kit
The Mouse Suppressors of Cytokine Signaling 1 (SOCS1) ELISA Kit is a powerful tool for the quantitative measurement of SOCS1 levels in mouse serum, plasma, and tissue lysates. This kit offers exceptional sensitivity and specificity, ensuring accurate and precise results for your research needs.SOCS1 is a key regulator of cytokine signaling pathways, playing a critical role in modulating immune responses and inflammation. Dysregulation of SOCS1 has been implicated in a variety of diseases, including autoimmune disorders, infectious diseases, and cancer.
By measuring SOCS1 levels, researchers can gain valuable insights into disease pathogenesis and potentially identify new therapeutic targets.Whether investigating the function of SOCS1 in immune regulation or exploring its potential as a diagnostic marker or therapeutic target, the Mouse SOCS1 ELISA Kit provides a reliable and easy-to-use solution for your research needs. Upgrade your research today with this cutting-edge ELISA kit from Assay Genie.
Product Name:
Mouse Suppressors Of Cytokine Signaling 1 (SOCS1) ELISA Kit
Product Code:
MODL01210
Size:
96 Assays
Target:
SOCS1
Synonyms:
CIS1, CISH1, JAB, SOCS-1, SSI-1, SSI1, TIP3, JAK-binding protein, STAT-induced STAT inhibitor 1, Tec-interacting protein 3
Detection Method:
Sandwich
Range:
0.312-20ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
