Mouse Surfactant Associated Protein B (SPB) ELISA Kit
The Mouse Surfactant Associated Protein B (SPB) ELISA Kit is a reliable and sensitive tool for the quantitative detection of SPB levels in mouse serum, plasma, and cell lysates. This kit offers high specificity and accuracy, ensuring consistent and precise results for a variety of research applications.Surfactant Associated Protein B (SPB) is a vital component of pulmonary surfactant, playing a crucial role in maintaining proper lung function and preventing respiratory distress. Abnormalities in SPB levels have been linked to various lung diseases, making it a valuable biomarker for studying respiratory conditions and developing targeted therapies.
The Mouse SPB ELISA Kit is easy to use and provides rapid results, making it a convenient option for researchers looking to investigate the role of SPB in lung function and disease. Its high sensitivity and reliability make it an ideal choice for laboratories conducting studies on pulmonary health and related disorders.
Product Name:
Mouse Surfactant Associated Protein B (SPB) ELISA Kit
Product Code:
MODL01219
Size:
96 Assays
Target:
SPB
Synonyms:
SFTPB, PSPB, SFTB3, SFTP3, SP-B, Pulmonary Surfactant Protein B, 18 kDa pulmonary-surfactant protein, 6 kDa protein
Detection Method:
Sandwich
Range:
0.781-50ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-99
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
