The Mouse SYT1 (Synaptotagmin-1) CLIA Kit is a highly sensitive and specific assay designed for the accurate detection of SYT1 levels in mouse samples, including serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it ideal for various research applications.SYT1, also known as synaptotagmin-1, plays a crucial role in neurotransmitter release at synapses, making it a key protein in neuronal communication.
Dysregulation of SYT1 has been implicated in various neurological disorders, making it an important biomarker for studying these conditions and developing potential therapies.Overall, the Mouse SYT1 CLIA Kit is a valuable tool for researchers studying synaptic transmission, neurological diseases, and potential therapeutic interventions.
Product Name:
Mouse SYT1 (Synaptotagmin 1) CLIA Kit
SKU:
MOES00543
Target:
Mouse SYT1 (Synaptotagmin 1)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse SYT1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse SYT1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse SYT1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse SYT1. You can calculate the concentration of Mouse SYT1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
102-114
99-115
101-119
Average (%)
108
107
109
1:4
Range (%)
93-107
84-96
86-97
Average (%)
98
91
91
1:8
Range (%)
86-97
85-100
95-108
Average (%)
92
92
101
1:16
Range (%)
87-101
101-114
95-108
Average (%)
95
108
102
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-104
95
EDTA plasma (n=5)
87-103
94
Cell culture media (n=5)
90-104
97
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
102.77
234.78
956.67
104.7
226.02
978.18
Standard deviation
12.49
16.97
66.2
10.38
20.34
74.44
C V (%)
12.15
7.23
6.92
9.91
9.0
7.61
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse SYT1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse SYT1 in samples. No significant cross-reactivity or interference between Mouse SYT1 and analogues was observed.
