The Mouse TACR1 (Tachykinin Receptor 1) CLIA Kit is specifically designed for the accurate measurement of TACR1 levels in mouse serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring reliable and reproducible results for various research applications.TACR1, also known as the tachykinin receptor 1, plays a critical role in the transmission of pain signals and inflammation in the body.
By accurately measuring TACR1 levels, researchers can gain valuable insights into the mechanisms underlying pain and inflammation, as well as potential therapeutic targets for related conditions.The Mouse TACR1 CLIA Kit is a valuable tool for studying pain, inflammation, and related disorders in mouse models, offering researchers a reliable and efficient method for quantifying TACR1 levels in biological samples.
Product Name:
Mouse TACR1 (Tachykinin Receptor 1) CLIA Kit
SKU:
MOES00546
Target:
Mouse TACR1 (Tachykinin Receptor 1)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse TACR1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse TACR1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse TACR1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse TACR1. You can calculate the concentration of Mouse TACR1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
96-112
93-107
100-115
Average (%)
103
100
106
1:4
Range (%)
95-108
100-117
96-111
Average (%)
102
109
103
1:8
Range (%)
89-103
95-108
85-97
Average (%)
94
101
91
1:16
Range (%)
95-109
85-96
92-103
Average (%)
102
91
97
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
98-112
106
EDTA plasma (n=5)
94-110
102
Cell culture media (n=5)
84-98
91
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
48.28
96.77
391.38
45.57
87.77
399.97
Standard deviation
4.9
7.87
35.54
3.67
8.79
35.48
C V (%)
10.15
8.13
9.08
8.05
10.01
8.87
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse TACR1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse TACR1 in samples. No significant cross-reactivity or interference between Mouse TACR1 and analogues was observed.
