The Mouse TACR2 (Tachykinin Receptor 2) CLIA Kit is a powerful tool for the quantitative measurement of TACR2 levels in mouse serum, plasma, and tissue homogenates. With its high sensitivity and specificity, this kit provides accurate and reliable results, making it perfect for a variety of research applications.Tachykinin Receptor 2 is a key receptor involved in neurotransmission and inflammatory responses, making it an important target for studying conditions such as pain, asthma, and gastrointestinal disorders.
By accurately measuring TACR2 levels, researchers can gain valuable insights into the role of this receptor in various biological processes and develop potential therapeutic interventions.Overall, the Mouse TACR2 CLIA Kit offers researchers a valuable tool for studying Tachykinin Receptor 2 function and its implications in health and disease. With its high-quality components and easy-to-use protocols, this kit is a must-have for any laboratory conducting research in the field of neuroscience and immunology.
Product Name:
Mouse TACR2 (Tachykinin Receptor 2) CLIA Kit
SKU:
MOES00547
Target:
Mouse TACR2 (Tachykinin Receptor 2)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse TACR2. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse TACR2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse TACR2, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse TACR2. You can calculate the concentration of Mouse TACR2 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
86-100
97-112
86-100
Average (%)
93
104
93
1:4
Range (%)
85-100
88-103
91-103
Average (%)
92
94
96
1:8
Range (%)
99-116
85-100
89-104
Average (%)
106
92
95
1:16
Range (%)
97-111
100-116
90-102
Average (%)
103
108
97
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-102
95
EDTA plasma (n=5)
90-100
95
Cell culture media (n=5)
91-105
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
103.79
241.46
780.88
105.95
245.91
748.97
Standard deviation
8.83
23.93
48.1
12.37
17.36
68.61
C V (%)
8.51
9.91
6.16
11.68
7.06
9.16
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse TACR2 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse TACR2 in samples. No significant cross-reactivity or interference between Mouse TACR2 and analogues was observed.
