The Mouse TFF2 (Trefoil Factor 2) CLIA Kit is specifically designed for the quantitative detection of TFF2 levels in mouse serum, plasma, and tissue homogenates. This kit offers exceptional sensitivity and accuracy, providing researchers with reliable and reproducible results for various research applications.TFF2 is a key protein involved in mucosal protection and repair, playing a critical role in maintaining gastrointestinal health. Dysregulation of TFF2 has been linked to various gastrointestinal disorders, making it a valuable biomarker for studying these conditions and developing targeted therapies.
With its ease of use and high performance, the Mouse TFF2 CLIA Kit is an indispensable tool for researchers investigating the role of TFF2 in health and disease. Its advanced technology and robust design ensure precise and dependable results, making it an essential addition to any laboratory studying gastrointestinal physiology and pathology.
Product Name:
Mouse TFF2 (Trefoil Factor 2) CLIA Kit
SKU:
MOES00579
Target:
Mouse TFF2 (Trefoil Factor 2)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse TFF2. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse TFF2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse TFF2, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse TFF2. You can calculate the concentration of Mouse TFF2 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
103-119
85-97
101-120
Average (%)
109
92
109
1:4
Range (%)
100-115
85-99
93-108
Average (%)
107
91
101
1:8
Range (%)
90-102
101-117
93-106
Average (%)
96
109
100
1:16
Range (%)
100-116
103-115
85-100
Average (%)
107
109
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-105
97
EDTA plasma (n=5)
89-102
95
Cell culture media (n=5)
94-108
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
46.13
105.0
460.81
44.97
108.12
458.47
Standard deviation
4.39
7.98
36.77
4.94
11.45
39.52
C V (%)
9.52
7.6
7.98
10.99
10.59
8.62
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse TFF2 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse TFF2 in samples. No significant cross-reactivity or interference between Mouse TFF2 and analogues was observed.
