Mouse TFR2 (Transferrin Receptor Protein 2) CLIA Kit
The Mouse TFR2 (Transferrin Receptor Protein 2) CLIA Kit is a highly sensitive assay designed for the accurate detection of TFR2 levels in mouse serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it an ideal tool for research in various fields.TFR2 is a key protein involved in iron metabolism and regulation, playing a crucial role in conditions such as hemochromatosis and anemia.
Monitoring TFR2 levels can provide valuable insights into these disorders and help in the development of targeted therapies.With its high specificity and ease of use, the Mouse TFR2 CLIA Kit is a valuable resource for researchers studying iron metabolism and related disorders in mouse models. Get accurate and reliable results with this innovative assay kit from Assay Genie.
Product Name:
Mouse TFR2 (Transferrin Receptor Protein 2) CLIA Kit
SKU:
MOES00572
Target:
Mouse TFR2 (Transferrin Receptor Protein 2)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse TFR2. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse TFR2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse TFR2, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse TFR2. You can calculate the concentration of Mouse TFR2 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
100-117
91-105
84-97
Average (%)
107
98
91
1:4
Range (%)
92-105
87-98
96-113
Average (%)
98
93
103
1:8
Range (%)
98-110
85-99
95-107
Average (%)
103
91
100
1:16
Range (%)
99-118
93-108
102-119
Average (%)
107
99
109
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-104
98
EDTA plasma (n=5)
93-109
101
Cell culture media (n=5)
92-107
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.93
3.15
7.88
0.85
2.92
8.1
Standard deviation
0.09
0.22
0.89
0.08
0.24
0.53
C V (%)
9.68
6.98
11.29
9.41
8.22
6.54
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse TFR2 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse TFR2 in samples. No significant cross-reactivity or interference between Mouse TFR2 and analogues was observed.
