The Mouse TGF-Alpha (Transforming Growth Factor-Alpha) CLIA Kit is designed for the accurate quantification of TGF-Alpha levels in mouse serum, plasma, and tissue homogenates. This kit offers high sensitivity and specificity, ensuring precise and reliable results for a variety of research applications.TGF-Alpha is a key growth factor involved in cell proliferation, differentiation, and survival. It plays a crucial role in various physiological processes, including tissue development, wound healing, and immune response regulation.
Aberrant TGF-Alpha levels have been linked to numerous diseases, such as cancer, inflammatory disorders, and developmental abnormalities, making this kit an essential tool for studying these conditions and potential therapeutic interventions.Overall, the Mouse TGF-Alpha CLIA Kit provides researchers with a valuable tool for investigating the role of TGF-Alpha in health and disease, offering reliable and reproducible results for advanced research studies.
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse TGF-α. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse TGF-α and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse TGF-α, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse TGF-α. You can calculate the concentration of Mouse TGF-α in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
85-97
100-119
92-106
Average (%)
90
108
97
1:4
Range (%)
92-108
97-113
97-110
Average (%)
99
104
103
1:8
Range (%)
92-105
92-103
98-114
Average (%)
99
97
104
1:16
Range (%)
98-109
90-104
99-112
Average (%)
103
95
105
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-105
98
EDTA plasma (n=5)
102-113
108
Cell culture media (n=5)
95-112
103
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
23.31
47.53
212.38
24.39
51.03
215.67
Standard deviation
2.47
5.03
13.19
2.68
4.22
14.86
C V (%)
10.6
10.58
6.21
10.99
8.27
6.89
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse TGF-α concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse TGF-α in samples. No significant cross-reactivity or interference between Mouse TGF-α and analogues was observed.
