Mouse TIMP-1 (Tissue Inhibitors of Metalloproteinase 1) CLIA Kit
The Mouse TIMP-1 (Tissue Inhibitors of Metalloproteinase-1) CLIA Kit is a comprehensive solution for the quantification of TIMP-1 levels in mouse samples, including serum, plasma, and tissue lysates. This kit offers exceptional sensitivity and specificity, guaranteeing accurate and consistent results for your research needs.TIMP-1 is a key regulator of metalloproteinases, enzymes that play a crucial role in tissue remodeling and extracellular matrix degradation. Dysregulation of TIMP-1 has been implicated in various diseases, including cancer, arthritis, and cardiovascular disorders, highlighting its importance as a potential therapeutic target and diagnostic marker.
With the Mouse TIMP-1 CLIA Kit, researchers can confidently analyze TIMP-1 levels in biological samples, providing valuable insights into disease mechanisms and potential treatment strategies. This kit is an invaluable tool for studies investigating the role of TIMP-1 in various physiological and pathological processes in mouse models.
Product Name:
Mouse TIMP-1 (Tissue Inhibitors of Metalloproteinase 1) CLIA Kit
SKU:
MOES00344
Target:
Mouse TIMP-1 (Tissue Inhibitors of Metalloproteinase 1)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse TIMP-1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse TIMP-1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse TIMP-1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse TIMP-1. You can calculate the concentration of Mouse TIMP-1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
94-109
84-98
102-115
Average (%)
100
91
109
1:4
Range (%)
103-115
92-108
100-117
Average (%)
109
98
107
1:8
Range (%)
88-104
94-110
98-111
Average (%)
95
100
105
1:16
Range (%)
103-117
86-99
99-112
Average (%)
109
92
106
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
99-115
107
EDTA plasma (n=5)
86-98
92
Cell culture media (n=5)
90-106
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
53.79
103.28
349.1
57.28
108.36
314.95
Standard deviation
6.23
8.07
28.07
6.08
10.71
20.72
C V (%)
11.58
7.81
8.04
10.61
9.88
6.58
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse TIMP-1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse TIMP-1 in samples. No significant cross-reactivity or interference between Mouse TIMP-1 and analogues was observed.
