Mouse TIMP-3 (Tissue Inhibitors of Metalloproteinase 3) CLIA Kit
The Mouse TIMP-3 (Tissue Inhibitors of Metalloproteinase-3) CLIA Kit is a valuable tool for researchers studying the role of TIMP-3 in various biological processes. This kit is specifically designed for the quantitative measurement of TIMP-3 levels in mouse tissue samples, providing highly accurate and reliable results.TIMP-3 is a key inhibitor of metalloproteinases, enzymes that play important roles in tissue remodeling, cell migration, and angiogenesis. Dysregulation of TIMP-3 has been implicated in various diseases, including cancer and cardiovascular disorders. By accurately measuring TIMP-3 levels, researchers can gain valuable insights into the underlying mechanisms of these diseases and potentially identify novel therapeutic targets.
The Mouse TIMP-3 CLIA Kit offers high sensitivity and specificity, ensuring reproducible results and facilitating detailed investigations into the functions of TIMP-3 in different experimental settings. With its user-friendly protocols and robust performance, this kit is an indispensable tool for advancing research in the fields of cancer biology, cardiovascular research, and tissue remodeling.
Product Name:
Mouse TIMP-3 (Tissue Inhibitors of Metalloproteinase 3) CLIA Kit
SKU:
MOES00568
Target:
Mouse TIMP-3 (Tissue Inhibitors of Metalloproteinase 3)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse TIMP-3. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse TIMP-3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse TIMP-3, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse TIMP-3. You can calculate the concentration of Mouse TIMP-3 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-100
91-101
94-110
Average (%)
92
96
101
1:4
Range (%)
93-105
83-98
96-108
Average (%)
99
90
102
1:8
Range (%)
99-115
93-105
89-101
Average (%)
105
99
95
1:16
Range (%)
91-106
92-104
98-116
Average (%)
98
97
106
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-108
100
EDTA plasma (n=5)
96-110
104
Cell culture media (n=5)
92-107
100
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
101.4
173.93
673.02
101.39
162.64
621.17
Standard deviation
12.79
13.27
58.69
11.41
19.34
45.16
C V (%)
12.61
7.63
8.72
11.25
11.89
7.27
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse TIMP-3 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse TIMP-3 in samples. No significant cross-reactivity or interference between Mouse TIMP-3 and analogues was observed.
