The Mouse TM (Thrombomodulin) CLIA Kit is a powerful tool for the quantitative measurement of thrombomodulin levels in mouse serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit delivers accurate and reproducible results, making it an invaluable resource for researchers in various fields. Thrombomodulin is a key protein involved in the regulation of coagulation and inflammation, playing a crucial role in maintaining vascular health.
Dysregulation of thrombomodulin has been implicated in various diseases, including sepsis, thrombosis, and inflammatory disorders, making it a promising biomarker for studying these conditions and potential therapeutic interventions. With the Mouse TM (Thrombomodulin) CLIA Kit, researchers can better understand the role of thrombomodulin in disease pathology and explore its potential as a target for novel treatments. Order your kit today and take your research to the next level.
Product Name:
Mouse TM (Thrombomodulin) CLIA Kit
SKU:
MOES00559
Target:
Mouse TM (Thrombomodulin)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse TM. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse TM and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse TM, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse TM. You can calculate the concentration of Mouse TM in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-99
88-100
92-104
Average (%)
93
95
98
1:4
Range (%)
100-112
104-118
85-98
Average (%)
106
110
91
1:8
Range (%)
93-106
103-115
95-109
Average (%)
99
109
101
1:16
Range (%)
93-108
91-107
101-115
Average (%)
100
97
109
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
100-115
107
EDTA plasma (n=5)
100-116
107
Cell culture media (n=5)
97-110
104
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
48.18
156.77
423.85
47.65
159.35
462.91
Standard deviation
5.32
12.54
47.85
4.07
13.1
28.84
C V (%)
11.04
8.0
11.29
8.54
8.22
6.23
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse TM concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse TM in samples. No significant cross-reactivity or interference between Mouse TM and analogues was observed.
