The Mouse TNFRSF1A (Tumor Necrosis Factor Receptor Superfamily Member 1A) CLIA Kit is a highly sensitive and specific assay designed for the precise measurement of TNFRSF1A levels in mouse serum, plasma, and cell culture supernatants. This kit is reliable and reproducible, making it perfect for various research applications.TNFRSF1A is a vital receptor involved in mediating the effects of tumor necrosis factor, impacting inflammatory responses and immune regulation.
Dysregulation of TNFRSF1A is implicated in various diseases such as autoimmune disorders, inflammatory conditions, and cancer. This kit provides a valuable tool for studying TNFRSF1A biology and potential therapeutic interventions.
Mouse TNFRSF1A (Tumor Necrosis Factor Receptor Superfamily, Member 1A)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse TNFRSF1A. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse TNFRSF1A and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse TNFRSF1A, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse TNFRSF1A. You can calculate the concentration of Mouse TNFRSF1A in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
98-112
85-95
103-117
Average (%)
103
90
109
1:4
Range (%)
95-108
103-118
97-111
Average (%)
101
110
102
1:8
Range (%)
85-99
94-107
89-105
Average (%)
92
101
96
1:16
Range (%)
98-112
96-113
85-98
Average (%)
106
103
90
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
96-108
101
EDTA plasma (n=5)
103-116
108
Cell culture media (n=5)
86-96
91
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
25.43
77.83
183.88
25.18
81.17
181.57
Standard deviation
2.57
6.13
19.53
2.05
8.92
14.31
C V (%)
10.11
7.88
10.62
8.14
10.99
7.88
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse TNFRSF1A concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse TNFRSF1A in samples. No significant cross-reactivity or interference between Mouse TNFRSF1A and analogues was observed.
