The Mouse TNFRSF1A (Tumor Necrosis Factor Receptor Superfamily, Member 1A) ELISA Kit is a specialized assay crafted for the precise and quantitative detection of TNFRSF1A in various biological samples. TNFRSF1A is a pivotal member of the tumor necrosis factor receptor superfamily, playing a crucial role in modulating cellular responses to inflammatory cytokines, particularly tumor necrosis factor-alpha (TNF-α). As a central mediator of TNF-α signaling, TNFRSF1A is involved in regulating inflammation, immune responses, cell survival, and apoptosis.
Accurate quantification of TNFRSF1A levels is essential for understanding its intricate functions in cellular processes, immune regulation, and disease pathogenesis. By measuring TNFRSF1A expression levels, researchers can gain valuable insights into the mechanisms underlying inflammatory conditions, autoimmune disorders, and infectious diseases where TNF-α signaling pathways are implicated. Driven by a commitment to quality and precision, the Mouse TNFRSF1A ELISA Kit from Assay Genie delivers exceptional sensitivity and specificity, ensuring reproducible and reliable results. Manufactured under stringent quality control measures, this kit offers robust performance and user-friendly protocols, making it an ideal choice for research applications aimed at unraveling the intricate roles of TNFRSF1A in biological systems.
Mouse TNFRSF1A (Tumor Necrosis Factor Receptor Superfamily, Member 1A)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse TNFRSF1A. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse TNFRSF1A and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse TNFRSF1A, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse TNFRSF1A. You can calculate the concentration of Mouse TNFRSF1A in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
91-104
94-108
90-100
Average (%)
96
101
95
1:4
Range (%)
88-101
92-107
85-98
Average (%)
95
100
92
1:8
Range (%)
91-108
93-108
86-100
Average (%)
98
100
91
1:16
Range (%)
88-99
96-113
90-103
Average (%)
93
103
96
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-107
100
EDTA plasma (n=5)
89-100
94
Cell culture media (n=5)
84-96
91
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
23.58
56.29
239.92
25.52
60.4
260.67
Standard deviation
1.41
3.23
10.77
1.62
2.53
11.29
C V (%)
5.96
5.74
4.49
6.33
4.19
4.33
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse TNFRSF1A concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse TNFRSF1A in samples. No significant cross-reactivity or interference between Mouse TNFRSF1A and analogues was observed.
