Mouse TNNI3/cTn-I (Troponin I Type 3, Cardiac) CLIA Kit
The Mouse TNNI3 (CTN I, Troponin I, Type 3, Cardiac) CLIA Kit is expertly designed for the precise measurement of troponin I levels in mouse serum, plasma, and cell culture supernatants. This innovative kit offers exceptional sensitivity and specificity, ensuring consistently accurate and reproducible results suitable for a diverse range of research needs.Troponin I, a vital cardiac protein, is integral in regulating muscle contraction and is a crucial biomarker for detecting cardiac injury or disease.
Monitoring troponin I levels can provide valuable insights into cardiac health and function, making this kit indispensable for investigating cardiovascular conditions and potential therapeutic strategies.Don't miss out on the opportunity to advance your research with the Mouse TNNI3 CLIA Kit – the key to unlocking new discoveries in cardiac biology and disease mechanisms.
Product Name:
Mouse TNNI3/cTn-I (Troponin I Type 3, Cardiac) CLIA Kit
SKU:
MOES00581
Target:
Mouse TNNI3/cTn-I (Troponin I Type 3, Cardiac)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
1.88 pg/mL
Detection range:
3.13-200 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse TNNI3/cTn-I. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse TNNI3/cTn-I and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse TNNI3/cTn-I, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse TNNI3/cTn-I. You can calculate the concentration of Mouse TNNI3/cTn-I in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
97-115
89-101
90-104
Average (%)
105
96
95
1:4
Range (%)
86-99
103-119
94-108
Average (%)
91
109
101
1:8
Range (%)
97-110
88-104
86-100
Average (%)
103
95
93
1:16
Range (%)
90-103
95-108
100-116
Average (%)
96
100
107
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-109
101
EDTA plasma (n=5)
92-108
100
Cell culture media (n=5)
94-109
100
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
10.73
18.04
72.27
11.34
18.22
78.21
Standard deviation
0.86
1.72
6.64
1.22
2.07
7.36
C V (%)
8.01
9.53
9.19
10.76
11.36
9.41
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse TNNI3/cTn-I concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse TNNI3/cTn-I in samples. No significant cross-reactivity or interference between Mouse TNNI3/cTn-I and analogues was observed.
