The Mouse Toll-Like Receptor 4 (TLR4) ELISA Kit is a reliable and sensitive assay designed for the accurate quantification of TLR4 levels in mouse serum, plasma, and cell culture supernatants. With its high specificity and precision, this kit provides researchers with consistent and reproducible results, making it an ideal tool for a variety of experimental studies.TLR4 is a key receptor involved in the innate immune response, playing a crucial role in detecting and responding to pathogen-associated molecular patterns. Dysregulation of TLR4 signaling has been linked to various inflammatory diseases, making it a valuable target for therapeutic interventions.
By measuring TLR4 levels, researchers can gain valuable insights into the immune response and potentially identify new strategies for combating infectious and inflammatory conditions.Overall, the Mouse Toll-Like Receptor 4 (TLR4) ELISA Kit is a valuable tool for studying immune responses and inflammatory pathways in mouse models, offering researchers a reliable method for characterizing TLR4 expression levels and exploring its role in various disease processes.
Product Name:
Mouse Toll Like Receptor 4 (TLR4) ELISA Kit
Product Code:
MODL01292
Size:
96 Assays
Target:
TLR4
Synonyms:
CD284, TOLL, HToll
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
