The Mouse TP53 (Tumor Protein 53) CLIA Kit is a highly reliable and sensitive assay designed for the accurate measurement of TP53 levels in mouse samples such as serum, plasma, and cell culture supernatants. This kit provides consistent and reproducible results, making it an excellent choice for a wide range of research applications.TP53, also known as Tumor Protein 53, is a key tumor suppressor protein that plays a critical role in regulating cell growth and preventing cancer development.
Dysregulation of TP53 is commonly associated with various types of cancer, making it a valuable biomarker for studying cancer progression and identifying potential therapeutic targets.With its high specificity and sensitivity, the Mouse TP53 CLIA Kit offers researchers a powerful tool for investigating the role of TP53 in cancer biology and developing novel approaches for cancer treatment. Unlock new insights into cancer biology with the Mouse TP53 CLIA Kit.
Product Name:
Mouse TP53 (Tumor Protein 53) CLIA Kit
SKU:
MOES00585
Target:
Mouse TP53 (Tumor Protein 53)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse TP53. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse TP53 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse TP53, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse TP53. You can calculate the concentration of Mouse TP53 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
94-107
91-103
91-106
Average (%)
100
97
99
1:4
Range (%)
101-117
92-103
99-115
Average (%)
109
97
105
1:8
Range (%)
85-99
97-112
104-116
Average (%)
91
103
110
1:16
Range (%)
100-118
97-113
92-107
Average (%)
108
105
99
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-106
98
EDTA plasma (n=5)
86-101
93
Cell culture media (n=5)
89-102
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
54.74
91.82
364.25
57.1
99.3
400.5
Standard deviation
6.36
7.42
39.81
4.65
10.23
30.8
C V (%)
11.62
8.08
10.93
8.14
10.3
7.69
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse TP53 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse TP53 in samples. No significant cross-reactivity or interference between Mouse TP53 and analogues was observed.
