The Mouse TRACP-5b (Tartrate-Resistant Acid Phosphatase 5b) CLIA Kit is a reliable and sensitive assay designed for the accurate measurement of TRACP-5b levels in mouse samples, including serum, plasma, and cell culture supernatants. This kit offers high specificity and reproducibility, ensuring precise results for a variety of research applications.TRACP-5b is an enzyme predominantly found in osteoclasts and is considered a marker for osteoclast number and activity.
It plays a crucial role in bone metabolism and remodeling, making it a valuable biomarker for studying bone-related diseases such as osteoporosis, osteopetrosis, and bone metastases.With its high sensitivity and specificity, the Mouse TRACP-5b CLIA Kit is an essential tool for researchers studying bone biology and related conditions, providing accurate measurements for a deeper understanding of osteoclast function and bone health.
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse TRACP-5b. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse TRACP-5b and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse TRACP-5b, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse TRACP-5b. You can calculate the concentration of Mouse TRACP-5b in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
101-118
100-114
95-108
Average (%)
108
108
101
1:4
Range (%)
91-105
98-114
102-115
Average (%)
96
105
109
1:8
Range (%)
93-109
101-117
83-95
Average (%)
99
108
90
1:16
Range (%)
91-105
90-100
98-113
Average (%)
98
95
105
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-100
92
EDTA plasma (n=5)
86-100
91
Cell culture media (n=5)
85-97
91
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.49
1.22
4.86
0.52
1.33
4.72
Standard deviation
0.05
0.09
0.3
0.06
0.15
0.52
C V (%)
10.2
7.38
6.17
11.54
11.28
11.02
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse TRACP-5b concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse TRACP-5b in samples. No significant cross-reactivity or interference between Mouse TRACP-5b and analogues was observed.
