The Mouse TRAIL (TNFSF10) Tumor Necrosis Factor-Related Apoptosis-Inducing Ligand CLIA Kit - MOES00536 is a highly sensitive and specific assay designed for the quantitative detection of TRAIL levels in mouse serum, plasma, and cell culture supernatants. This kit provides accurate and reproducible results, making it ideal for various research applications.TRAIL, also known as TNFSF10, is a critical protein involved in apoptosis induction and immune response regulation. Its dysregulation has been implicated in various diseases, including cancer and autoimmune disorders.
Therefore, measuring TRAIL levels can provide valuable insights into disease progression and potential therapeutic strategies.Overall, the Mouse TRAIL (TNFSF10) Tumor Necrosis Factor-Related Apoptosis-Inducing Ligand CLIA Kit - MOES00536 is a valuable tool for researchers studying the role of TRAIL in health and disease. Its high sensitivity and specificity ensure reliable results, making it a key component in experimental studies and drug development efforts.
Mouse TRAIL/TNFSF10 (Tumor Necrosis Factor Related Apoptosis Inducing Ligand)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse TRAIL/TNFSF10. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse TRAIL/TNFSF10 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse TRAIL/TNFSF10, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse TRAIL/TNFSF10. You can calculate the concentration of Mouse TRAIL/TNFSF10 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
94-108
103-117
98-110
Average (%)
100
110
104
1:4
Range (%)
89-106
100-113
97-112
Average (%)
96
107
105
1:8
Range (%)
92-109
98-115
100-114
Average (%)
99
105
108
1:16
Range (%)
96-108
88-102
101-116
Average (%)
103
95
109
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
97-114
104
EDTA plasma (n=5)
92-104
97
Cell culture media (n=5)
88-104
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
26.36
43.96
246.5
25.88
43.18
264.92
Standard deviation
2.93
5.16
17.95
2.85
4.59
28.72
C V (%)
11.12
11.74
7.28
11.01
10.63
10.84
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse TRAIL/TNFSF10 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse TRAIL/TNFSF10 in samples. No significant cross-reactivity or interference between Mouse TRAIL/TNFSF10 and analogues was observed.
