Mouse TREM-1 (Triggering Receptor Expresses on Myeloid Cells-1) CLIA Kit
The Mouse TREM-1 (Triggering Receptor Expressed on Myeloid Cells-1) CLIA Kit is specifically designed for the accurate and sensitive detection of TREM-1 levels in mouse samples such as serum, plasma, and cell lysates. This kit offers high sensitivity and specificity, providing reliable and reproducible results for your research needs.TREM-1 is a key regulator of the immune response, particularly in inflammatory processes. Its role in various diseases such as sepsis, inflammatory bowel disease, and autoimmune disorders makes it a valuable biomarker for studying these conditions and developing therapeutic interventions.
With the Mouse TREM-1 CLIA Kit, you can confidently measure TREM-1 levels in mouse samples, advancing your research in immunology and inflammatory diseases. Trust in the precision and accuracy of this kit to support your scientific discoveries. Purchase yours today from Assay Genie.
Product Name:
Mouse TREM-1 (Triggering Receptor Expresses on Myeloid Cells-1) CLIA Kit
SKU:
MOES00347
Target:
Mouse TREM-1 (Triggering Receptor Expresses on Myeloid Cells-1)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
1.88 pg/mL
Detection range:
3.13-200 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse TREM-1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse TREM-1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse TREM-1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse TREM-1. You can calculate the concentration of Mouse TREM-1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
98-114
101-114
93-108
Average (%)
107
109
99
1:4
Range (%)
90-103
98-111
89-100
Average (%)
96
106
95
1:8
Range (%)
101-112
97-112
94-108
Average (%)
107
105
100
1:16
Range (%)
104-120
88-99
101-115
Average (%)
110
94
109
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
102-115
107
EDTA plasma (n=5)
91-103
98
Cell culture media (n=5)
100-116
106
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
9.5
23.96
77.51
9.54
21.58
74.96
Standard deviation
1.16
1.7
8.08
0.81
1.98
8.19
C V (%)
12.21
7.1
10.42
8.49
9.18
10.93
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse TREM-1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse TREM-1 in samples. No significant cross-reactivity or interference between Mouse TREM-1 and analogues was observed.
