Mouse Troponin T Type 1, Slow Skeletal (TNNT1) ELISA Kit
The Mouse Troponin T Type 1 Slow Skeletal (TNNT1) ELISA Kit is specifically designed for the quantitative measurement of TNNT1 levels in mouse serum, plasma, and tissue homogenates. With a high level of accuracy and sensitivity, this kit provides reliable and reproducible results for research purposes.TNNT1 is a protein essential for muscle contraction and function, particularly in slow skeletal muscle fibers. Dysregulation of TNNT1 has been associated with skeletal muscle disorders and neuromuscular diseases, making it a valuable biomarker for studying these conditions and developing potential treatments.
This ELISA kit is a valuable tool for researchers studying muscle physiology, muscle disorders, and neuromuscular diseases in mouse models. It offers a comprehensive and reliable method for measuring TNNT1 levels, aiding in the advancement of research in this field.
Product Name:
Mouse Troponin T Type 1, Slow Skeletal (TNNT1) ELISA Kit
Product Code:
MODL01310
Size:
96 Assays
Target:
TNNT1
Synonyms:
ANM, STNT, TNT, TNTS, Slow Skeletal Muscle Troponin T
Detection Method:
Sandwich
Range:
15.625-1,000pg/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
