The Mouse TRX (Thioredoxin) CLIA Kit is specifically designed for the precise measurement of thioredoxin levels in mouse serum, plasma, and cell culture supernatants. This kit offers unparalleled sensitivity and accuracy, guaranteeing dependable and consistent results for a variety of research purposes.Thioredoxin is a vital protein that plays a key role in redox regulation and cell signaling, impacting various physiological processes such as cell growth, inflammation, and immune response. Abnormal thioredoxin levels have been linked to a range of diseases, including cancer, autoimmune disorders, and neurodegenerative conditions, underscoring its importance as a biomarker for disease progression and therapeutic development.
With the Mouse TRX (Thioredoxin) CLIA Kit from Assay Genie, researchers can confidently explore the intricate mechanisms of thioredoxin signaling and its implications in disease pathogenesis, paving the way for innovative diagnostic and therapeutic interventions.
Product Name:
Mouse Trx (Thioredoxin) CLIA Kit
SKU:
MOES00555
Target:
Mouse Trx (Thioredoxin)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse Trx. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse Trx and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse Trx, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse Trx. You can calculate the concentration of Mouse Trx in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
96-108
94-108
97-110
Average (%)
102
101
104
1:4
Range (%)
94-108
99-112
97-113
Average (%)
100
106
105
1:8
Range (%)
84-99
90-107
91-108
Average (%)
91
98
99
1:16
Range (%)
100-114
99-115
99-114
Average (%)
108
105
106
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
95-110
101
EDTA plasma (n=5)
90-101
95
Cell culture media (n=5)
84-96
91
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
52.17
93.85
448.69
53.64
91.71
417.16
Standard deviation
6.0
10.28
48.01
6.7
8.19
36.92
C V (%)
11.5
10.95
10.7
12.49
8.93
8.85
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse Trx concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse Trx in samples. No significant cross-reactivity or interference between Mouse Trx and analogues was observed.
