The Mouse Tryptophan Hydroxylase 1 (TPH1) ELISA Kit is a high-quality assay designed for the precise measurement of TPH1 levels in mouse serum, plasma, and tissue homogenates. This kit offers exceptional sensitivity and accuracy, ensuring consistent and reliable results for various research experiments.Tryptophan Hydroxylase 1 is a key enzyme involved in the synthesis of serotonin, a neurotransmitter that plays a crucial role in regulating mood, behavior, and cognition. Dysregulation of TPH1 has been implicated in various psychiatric disorders, including depression and anxiety, as well as in gastrointestinal disorders such as irritable bowel syndrome.
By accurately measuring TPH1 levels, researchers can gain valuable insights into the underlying mechanisms of these conditions and potentially develop novel therapeutic strategies. The Mouse TPH1 ELISA Kit provides a valuable tool for studying the role of this enzyme in health and disease, advancing our understanding of serotonin signaling pathways.
Product Name:
Mouse Tryptophan Hydroxylase 1 (TPH1) ELISA Kit
Product Code:
MODL01319
Size:
96 Assays
Target:
TPH1
Synonyms:
TPH, TPRH, TRPH, Tryptophan 5-Monooxygenase
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
