Mouse TSP-1 (Thrombin Sensitive Protein 1) CLIA Kit
The Mouse TSP-1 (Thrombin-Sensitive Protein-1) CLIA Kit is specifically designed for the quantitative detection of TSP-1 levels in mouse serum, plasma, and tissue homogenates. This kit offers high sensitivity and accuracy, ensuring precise and reliable results for a variety of research applications.TSP-1 is a key protein involved in regulating cell growth, adhesion, and apoptosis, making it essential for maintaining tissue homeostasis and angiogenesis.
Its dysregulation has been linked to various pathological conditions, including cancer, inflammation, and cardiovascular diseases, making it a valuable biomarker for studying these diseases and developing potential therapeutic interventions.Overall, the Mouse TSP-1 CLIA Kit provides researchers with a powerful tool for investigating the role of TSP-1 in disease progression and identifying potential targets for therapeutic intervention.
Product Name:
Mouse TSP-1 (Thrombin Sensitive Protein 1) CLIA Kit
SKU:
MOES00557
Target:
Mouse TSP-1 (Thrombin Sensitive Protein 1)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse TSP-1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse TSP-1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse TSP-1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse TSP-1. You can calculate the concentration of Mouse TSP-1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
98-114
88-104
102-113
Average (%)
105
95
108
1:4
Range (%)
94-106
95-109
88-98
Average (%)
100
103
93
1:8
Range (%)
96-108
94-111
88-100
Average (%)
102
102
94
1:16
Range (%)
84-98
86-98
89-103
Average (%)
91
91
95
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
84-98
90
EDTA plasma (n=5)
86-101
93
Cell culture media (n=5)
87-103
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
52.19
144.17
495.17
53.66
132.17
466.25
Standard deviation
5.16
10.47
40.85
6.09
10.72
41.22
C V (%)
9.89
7.26
8.25
11.35
8.11
8.84
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse TSP-1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse TSP-1 in samples. No significant cross-reactivity or interference between Mouse TSP-1 and analogues was observed.
