The Mouse TTR (Transthyretin) CLIA Kit is a powerful tool for measuring transthyretin levels in mouse serum, plasma, and tissue samples. With its high sensitivity and specificity, this kit provides accurate and reliable results for a variety of research applications.Transthyretin is a critical protein involved in transporting thyroid hormones and vitamin A in the body. Dysregulation of transthyretin levels has been linked to various conditions such as familial amyloid polyneuropathy and senile systemic amyloidosis.
By accurately measuring transthyretin levels, researchers can gain valuable insights into these diseases and explore potential treatment options.Whether studying neurodegenerative disorders, metabolic diseases, or other conditions related to transthyretin, the Mouse TTR CLIA Kit is an essential tool for researchers seeking to further their understanding of these complex diseases.
Product Name:
Mouse TTR (Transthyretin) CLIA Kit
SKU:
MOES00578
Target:
Mouse TTR (Transthyretin)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
0.94 ng/mL
Detection range:
1.56-100 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse TTR. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse TTR and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse TTR, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse TTR. You can calculate the concentration of Mouse TTR in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
100-116
92-104
101-118
Average (%)
108
97
107
1:4
Range (%)
95-112
88-99
89-102
Average (%)
102
93
95
1:8
Range (%)
90-104
90-100
91-105
Average (%)
96
95
96
1:16
Range (%)
100-113
88-100
91-106
Average (%)
105
94
98
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-101
92
EDTA plasma (n=5)
99-112
104
Cell culture media (n=5)
98-112
105
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
4.81
8.74
35.15
4.42
8.49
37.81
Standard deviation
0.52
1.04
2.58
0.43
0.89
3.55
C V (%)
10.81
11.9
7.34
9.73
10.48
9.39
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse TTR concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse TTR in samples. No significant cross-reactivity or interference between Mouse TTR and analogues was observed.
