The Mouse UCHL1 (Ubiquitin Carboxyl-Terminal Hydrolase L1) ELISA Kit is specifically designed for the accurate measurement of UCHL1 levels in mouse serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reproducible results, making it an ideal tool for a wide range of research applications.UCHL1 is a key enzyme involved in the regulation of ubiquitin processing and protein degradation, playing a crucial role in maintaining cellular homeostasis.
Abnormal UCHL1 expression has been linked to various diseases, including neurodegenerative disorders and cancer, making it a valuable biomarker for studying these conditions and exploring potential therapeutic interventions.Overall, the Mouse UCHL1 ELISA Kit provides researchers with a reliable and accurate method for quantifying UCHL1 levels in mouse samples, enabling in-depth investigations into the role of this enzyme in various physiological and pathological processes.
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse UCHL1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse UCHL1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse UCHL1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse UCHL1. You can calculate the concentration of Mouse UCHL1 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
97-112
96-113
84-95
Average (%)
103
103
90
1:4
Range (%)
90-101
80-91
85-100
Average (%)
95
86
92
1:8
Range (%)
89-102
81-94
87-102
Average (%)
95
87
94
1:16
Range (%)
93-108
79-93
85-100
Average (%)
99
86
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-102
96
EDTA plasma (n=5)
89-102
96
Cell culture media (n=5)
93-106
100
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
193.96
439.24
1375.45
187.35
410.81
1363.59
Standard deviation
12.59
22.93
42.91
11.63
16.97
69.68
C V (%)
6.49
5.22
3.12
6.21
4.13
5.11
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse UCHL1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse UCHL1 in samples. No significant cross-reactivity or interference between Mouse UCHL1 and analogues was observed.
