Mouse VAP-1 (Vascular Adhesion Protein 1) ELISA Kit
The Mouse VAP-1 (Vascular Adhesion Protein-1) ELISA Kit is specifically designed for the accurate quantification of VAP-1 levels in mouse serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and consistent results, making it an excellent tool for a variety of research applications.VAP-1 is a key protein involved in the adhesion of leukocytes to the endothelium, playing a crucial role in inflammatory processes and immune responses. Dysregulation of VAP-1 has been implicated in various diseases such as autoimmune disorders, diabetes, and cardiovascular diseases, making it a valuable biomarker for studying these conditions and developing potential therapeutic interventions.
The Mouse VAP-1 ELISA Kit from AssayGenie provides researchers with a reliable and efficient method to study the role of VAP-1 in health and disease, offering valuable insights into potential treatment strategies for a range of inflammatory and immune-related disorders.
Product Name:
Mouse VAP-1 (Vascular Adhesion Protein 1) ELISA Kit
SKU:
MOES00957
Target:
Mouse VAP-1 (Vascular Adhesion Protein 1)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse VAP-1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse VAP-1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse VAP-1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse VAP-1. You can calculate the concentration of Mouse VAP-1 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
96-113
89-102
90-103
Average (%)
103
96
96
1:4
Range (%)
88-101
86-97
83-96
Average (%)
94
91
89
1:8
Range (%)
89-102
80-90
86-102
Average (%)
95
85
93
1:16
Range (%)
91-106
81-92
83-94
Average (%)
98
86
89
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-99
93
EDTA plasma (n=5)
90-106
96
Cell culture media (n=5)
91-107
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.53
1.27
4.5
0.54
1.17
4.06
Standard deviation
0.03
0.07
0.18
0.03
0.06
0.17
C V (%)
5.66
5.51
4.0
5.56
5.13
4.19
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse VAP-1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse VAP-1 in samples. No significant cross-reactivity or interference between Mouse VAP-1 and analogues was observed.
